| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240379 |
| Chromosome: | chromosome 12 |
| Location: | 4182186 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTTACAGGACCGGTACCGTGTTACGC |
| Internal bar code: | TTCGACATCTCACGATTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 420 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTCTCCATAACGCACAG |
| Suggested primer 2: | CTGGTAGGGACTGTTGCCAT |