| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240386 |
| Chromosome: | chromosome 7 |
| Location: | 4917315 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346400 | FKM6 | DNA methylase in the FkbM family; (1 of 3) K05302 - SET domain-containing protein 6 (SETD6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGTGCCGCGCGCGCCCGCCCGCTTTC |
| Internal bar code: | TAGTAGTATCGGACGGAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 93.4461 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGAGTCAACTAGGCCG |
| Suggested primer 2: | CCTGACGCTGCTTTCCTAAC |