| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240404 |
| Chromosome: | chromosome 17 |
| Location: | 3553119 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725450 | (1 of 1) K11789 - HIV-1 Vpr-binding protein (VPRBP, DCAF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGGCACGCAGGTGCTTCGCCCCAGGT |
| Internal bar code: | CGAGACTACGAGAAGACGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTATCACTGGACCTGGCG |
| Suggested primer 2: | GGGCTCTCCAGCTCCTTACT |