Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.240412 |
Chromosome: | chromosome 2 |
Location: | 3880917 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g096000 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGAGGCGCGCACGCACGCAAGCACGC |
Internal bar code: | TACGTCACCGATGTGGTCGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 290 |
LEAP-Seq percent confirming: | 80.1653 |
LEAP-Seq n confirming: | 97 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAAACCATGTGCAAAACAC |
Suggested primer 2: | GAAGTGAGCTGCACCAATCA |