Insertion junction: LMJ.RY0402.240413_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.240413
Chromosome:chromosome 5
Location:782601
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g248300 NRAMP4 Manganese/metal transporter, NRAMP homolog; (1 of 1) K12347 - natural resistance-associated macrophage protein (SLC11A, NRAMP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATCTTCACACACGCCCACCCCACCCC
Internal bar code:CGTATGCAGACCGACTCCACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:424
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGATTAGGTGAGTTCGC
Suggested primer 2:GGATGTTTGGAAAAGCGGTA