Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.240423 |
Chromosome: | chromosome_12 |
Location: | 5584865 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g531800 | FAP7 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGAACACATCAACCACGTCAGCCTCCCA |
Internal bar code: | TTCGCGAGGTTCGGCTTAAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 465 |
LEAP-Seq percent confirming: | 78.934 |
LEAP-Seq n confirming: | 311 |
LEAP-Seq n nonconfirming: | 83 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGGTACCTAGCACTGCTTC |
Suggested primer 2: | GAAGCAGGATACGGGGTGTA |