| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240450 |
| Chromosome: | chromosome 17 |
| Location: | 478867 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699200 | (1 of 1) IPR000104//IPR000626//IPR029071 - Antifreeze protein, type I // Ubiquitin domain // Ubiquitin-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGAGCCGGTCCTCCCGCAGCCAACCGCA |
| Internal bar code: | TTTCGAGGGGCCAACTCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 4.39476 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 1240 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCTCACAGTTCCACA |
| Suggested primer 2: | GAAGAAGAGAGGACGCGATG |