| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240476 |
| Chromosome: | chromosome 1 |
| Location: | 6979017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050400 | TIM22C | Mitochondrial inner membrane translocase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAGTGCGAGTATGTTGTCGCTCGCAGC |
| Internal bar code: | CCTAATTACTGCTTCCTGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCGTTAGAGAGCACAA |
| Suggested primer 2: | TCCTGGGTCTTTGACTTTGG |