| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240489 |
| Chromosome: | chromosome 9 |
| Location: | 803168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401950 | (1 of 8) 2.7.11.12 - cGMP-dependent protein kinase / PKG II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAGAAGAGGACAGTAAGTCTGACGAAA |
| Internal bar code: | CTTTACGCAGACCAGCGGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAACCAGTGTCAGCGTT |
| Suggested primer 2: | AATAGTTCCAGACCCCACCC |