Insertion junction: LMJ.RY0402.240515_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.240515
Chromosome:chromosome 17
Location:896113
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g702700 TPT28,TPT27 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 1) PTHR11132//PTHR11132:SF101//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGAACACGTCACGCGCGAGAGGCTTTG
Internal bar code:CTACGGACAAGGATACGAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:568
LEAP-Seq percent confirming:91.4286
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGACTTCCTTTCAGACCG
Suggested primer 2:TGGGGATAGGAACGAGAATG