| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240532 |
| Chromosome: | chromosome_2 |
| Location: | 7347115 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTGTTCCAGCTGTCCGTCAACCTGGTGG |
| Internal bar code: | GATGGCGAACCAGGTCCCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 99.6933 |
| LEAP-Seq n confirming: | 325 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATGACTCATCGGGACA |
| Suggested primer 2: | AGGGCAGACGACAGAAGTGT |