Insertion junction: LMJ.RY0402.240532_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.240532
Chromosome:chromosome 3
Location:6483131
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g194900 CYA4 Lipocalin-like adenylate cyclase; (1 of 3) PTHR11017//PTHR11017:SF145//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAATTTTAATGACGCCTCGCAATGTACA
Internal bar code:TATCATCAGGGGGGTGACGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:609
LEAP-Seq percent confirming:98.9975
LEAP-Seq n confirming:395
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCATAAGGCATAGCTGGCTC
Suggested primer 2:ACGCTCTCTCTCTTTCGCTG