| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240532 |
| Chromosome: | chromosome_3 |
| Location: | 6483131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g194900 | CYA4 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAATTTTAATGACGCCTCGCAATGTACA |
| Internal bar code: | TATCATCAGGGGGGTGACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 98.9975 |
| LEAP-Seq n confirming: | 395 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAAGGCATAGCTGGCTC |
| Suggested primer 2: | ACGCTCTCTCTCTTTCGCTG |