| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240617 |
| Chromosome: | chromosome 7 |
| Location: | 4109866 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342920 | (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGTTTGTTTTTGTTTAACACACGCGCA |
| Internal bar code: | ATAGGGGCCCCTTTTTGGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.7704 |
| LEAP-Seq n confirming: | 869 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTCACCACCACAAATC |
| Suggested primer 2: | ACGCCCTTACTGTTTCATGG |