Insertion junction: LMJ.RY0402.240617_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.240617
Chromosome:chromosome 7
Location:4109893
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCAACAAATACCTACGCCGGTGCGCGTG
Internal bar code:CTTAGGCGCGAAGAACTGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:26.6949
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:173
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCTCACCACCACAAATC
Suggested primer 2:ACGCCCTTACTGTTTCATGG