| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240651 |
| Chromosome: | chromosome 13 |
| Location: | 2017841 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576800 | PHX21,HIF1,HIF | (1 of 1) K09592 - hypoxia-inducible factor prolyl hydroxylase (EGLN, HPH); Cytosolic prolyl 4-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATAAACCACTGCCAACCCAGAATCCAGG |
| Internal bar code: | GTGGCGTAAGGAACGTAATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCTGCCAGTTGTAATC |
| Suggested primer 2: | GATCGACAGGTGTGGTTGTG |