| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240660 |
| Chromosome: | chromosome 5 |
| Location: | 2555799 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236050 | (1 of 2) IPR015097 - Lung surfactant protein D coiled-coil trimerisation | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTATGGAACCGAAGTCAGATGCCGTGA |
| Internal bar code: | GTGCGCGGCTTGCGTCCGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.0017 |
| LEAP-Seq n confirming: | 595 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGTACCTGACACAGACT |
| Suggested primer 2: | CATGCTAGCTAGGACGGAGG |