| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240672 |
| Chromosome: | chromosome 13 |
| Location: | 2007520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576740 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATCAGCAAGGGCGGTGGGCAACTTGAC |
| Internal bar code: | AATGCTTAAGGGCGTCCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 99.6316 |
| LEAP-Seq n confirming: | 1893 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGGAGCTGAGAGGCAT |
| Suggested primer 2: | GGCTTGGCTAGGACTCACTG |