| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240690 |
| Chromosome: | chromosome 2 |
| Location: | 1028541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080550 | PDE26 | (1 of 1) PF00012//PF00233 - Hsp70 protein (HSP70) // 3'5'-cyclic nucleotide phosphodiesterase (PDEase_I); 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGACATATGCATATGGCTACGGTATGG |
| Internal bar code: | CCGCCCCCTCGCGCAGACTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 93.662 |
| LEAP-Seq n confirming: | 266 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTAGTTTGGGCTGGTA |
| Suggested primer 2: | GTGTATAGGCAACGGCAGGT |