| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240698 |
| Chromosome: | chromosome 17 |
| Location: | 333587 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698365 | PF7,CCDC40,FAP172 | Flagellar Associated Protein 172 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCCCCTGCTCTTCCCCTGCTATGCCC |
| Internal bar code: | TCACTATCTATGTGCAAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 95.283 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGTTGTCACTGGAGGA |
| Suggested primer 2: | TAGTTAGGGCAGGAGGAGCA |