| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240721 |
| Chromosome: | chromosome 12 |
| Location: | 5165502 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATGTAAAATTACCATATAGCGTCACA |
| Internal bar code: | AAATGGGGTATCCCCTCATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 90.7407 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGACATCCCTCCAGGAC |
| Suggested primer 2: | ACTGCCAAATGTTCGTGTCA |