| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240730 |
| Chromosome: | chromosome 16 |
| Location: | 5126381 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683850 | GDPD1,GDP1 | Glycerophosphoryl diester phosphodiesterase family protein; (1 of 5) PTHR23344//PTHR23344:SF7 - GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE // GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGAAGTCGGCGCCTGTGTAAGTGCAG |
| Internal bar code: | TAAGATTCCTTTGAATGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 99.2086 |
| LEAP-Seq n confirming: | 2758 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGTTCGGACCCATGCAC |
| Suggested primer 2: | CACTCCGCTTAAAGGTCTGC |