| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240739 |
| Chromosome: | chromosome 13 |
| Location: | 874735 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567500 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGGTCGTATCGCCGTCAACCCCGCCGC |
| Internal bar code: | CATGTGCAGCGACTCCAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 99.4368 |
| LEAP-Seq n confirming: | 2472 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCAGGTTCTTATTTGGC |
| Suggested primer 2: | GAGCACAGCCATGAGACGTA |