| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240745 |
| Chromosome: | chromosome 12 |
| Location: | 164835 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484600 | DMC6 | Putative DNA methylase; (1 of 1) 2.1.1.204 - tRNA (cytosine(38)-C(5))-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGATGGTGGTGTGGTCCGCATCCTGGAC |
| Internal bar code: | ATGCCGATTCCCCTAATGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCACCAGATGCAGTAG |
| Suggested primer 2: | TGTGGCTCCTCCTTAAATGG |