| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240794 |
| Chromosome: | chromosome 4 |
| Location: | 2477822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221770 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTAGCAGTCTGCCACCGGGCAGGTCAG |
| Internal bar code: | TCGCAGGTCCTAATTCCAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 93.8983 |
| LEAP-Seq n confirming: | 277 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAGGTCTGCCACAGCAC |
| Suggested primer 2: | GAGTAGCGCCAAGGCTATTG |