| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240836 |
| Chromosome: | chromosome 12 |
| Location: | 6060694 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535350 | (1 of 1) IPR011044//IPR017986 - Quinoprotein amine dehydrogenase, beta chain-like // WD40-repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACATTAGCCTGCACAATTTCGGACAC |
| Internal bar code: | GGCCGTCCCGCATCACCGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 99.1111 |
| LEAP-Seq n confirming: | 223 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGACAGCTCTTCATCGC |
| Suggested primer 2: | GATGTACATTGCAACACGGG |