| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240843 |
| Chromosome: | chromosome 16 |
| Location: | 1646940 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAATCCTTATTCATTCCATCGGTTTG |
| Internal bar code: | CTCGCCTCGAGTACGGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 82.9187 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTGGTACAAATTCGGC |
| Suggested primer 2: | TGATTGGACTGCAAGCGTAG |