| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240851 |
| Chromosome: | chromosome 12 |
| Location: | 7941370 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552350 | (1 of 4) PF04191 - Phospholipid methyltransferase (PEMT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTACAGTTGCGTAATGGAGCCGGCAAT |
| Internal bar code: | GAATTGTTCATGTTTAGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.5803 |
| LEAP-Seq n confirming: | 1898 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATGCGGAGGAGGATAAC |
| Suggested primer 2: | GTGCGTCATCCCATCTACCT |