| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240859 |
| Chromosome: | chromosome 11 |
| Location: | 509722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTGACATTGCGCACAAACAAACTACTC |
| Internal bar code: | GAAAGGAACGGCCTCGAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 97.686 |
| LEAP-Seq n confirming: | 591 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGATTTGCAACAGTAA |
| Suggested primer 2: | ACGCATGATAGGGGTACTGC |