| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240875 |
| Chromosome: | chromosome 6 |
| Location: | 3089568 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274700 | OTU4 | (1 of 2) K13719 - ubiquitin thioesterase OTU1 (OTU1, YOD1); OTU-like cysteine protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCGTGTTTGGTTAGCAGTACCCTTA |
| Internal bar code: | AGTCGGTTTAGGCAGGGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTTCGCTTCCACAGAT |
| Suggested primer 2: | TTGGTGATTAGCAGGTCGTG |