| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240877 |
| Chromosome: | chromosome 17 |
| Location: | 2801549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718850 | RFA1,RPA70A | (1 of 1) PTHR23273//PTHR23273:SF12 - REPLICATION FACTOR A 1, RFA1 // REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT C-RELATED; Replication protein A, 70 kDa DNA-binding subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGTAGCGTCAAACGCAGCAGTGGAT |
| Internal bar code: | GCATATCCTTGCGAGTGATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.4554 |
| LEAP-Seq n confirming: | 8948 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCCAATATGCGTTCAGC |
| Suggested primer 2: | ATAACCAGCATCAACAGGGC |