Insertion junction: LMJ.RY0402.240891_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.240891
Chromosome:chromosome 13
Location:2355266
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g579350 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGTCGGTTAACCAACAGAAACTGTGTC
Internal bar code:TCTGTGGGCGTTGAAACAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:544
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:180
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCCACCAACAAACTTGC
Suggested primer 2:TGCAGGCGCATGTTAGATAG