Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.240939 |
Chromosome: | chromosome 17 |
Location: | 5228648 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g735876 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTACGCATTTCTGATGCTGCGGCCGTTC |
Internal bar code: | TACGGGAGCGACCCTAGACGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 193 |
LEAP-Seq percent confirming: | 80.9783 |
LEAP-Seq n confirming: | 149 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCATACAGTGTGCGATGGG |
Suggested primer 2: | CTTGTGCAGACAACTCCGAA |