Insertion junction: LMJ.RY0402.240957_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.240957
Chromosome:chromosome 17
Location:5479953
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g737350 (1 of 32) IPR029062 - Class I glutamine amidotransferase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAAGTCATGCCGCGCGTCGATTTACGAC
Internal bar code:CGTGCTGCTAATTGTACGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:65.3846
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTTGAATGTTGGGGTC
Suggested primer 2:GCGTCTATCGCATCTTGTCA