| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240959 |
| Chromosome: | chromosome 8 |
| Location: | 1859714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGTGGTCATGCTGCTCCGCCGGCCGCT |
| Internal bar code: | AGGGGGCAAGGGGTGGTGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 94.1558 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACGTCAGAGGAGCAAG |
| Suggested primer 2: | GTTCGGTCGGCATTGATTAC |