| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240977 |
| Chromosome: | chromosome 9 |
| Location: | 3657599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390541 | (1 of 4) 3.1.1.23 - Acylglycerol lipase / Monoacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTTGAGGCCATGAACAAGGCGGGCTT |
| Internal bar code: | TCGCCCAACCACTATTAGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 97.8593 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAACTCCTCGACGTTGC |
| Suggested primer 2: | ATGTGCCCTGTTACTTTGGC |