| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240981 |
| Chromosome: | chromosome 6 |
| Location: | 6472068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292900 | (1 of 10) PTHR12270:SF6 - GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCGAGCTTGAGAGCATGTGAGAGCG |
| Internal bar code: | GGAGTGTCTCGGGACTCGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 99.3174 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGAAACCAAACGGAGTG |
| Suggested primer 2: | TATGCTTGGTTCCCACTTCC |