Insertion junction: LMJ.RY0402.241011_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241011
Chromosome:chromosome 10
Location:4911467
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g454850 (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATGGCACAACCGCACAGAAAGTTTAGGA
Internal bar code:TCGAAGGACAGGTAGGGAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:193
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTGTGCTTTCACGCTCTG
Suggested primer 2:ACACACCACACACACACACG