| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241023 |
| Chromosome: | chromosome 7 |
| Location: | 3312580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334600 | CGL20 | (1 of 1) PTHR35713//PTHR35713:SF1 - FAMILY NOT NAMED // GENOMIC DNA, CHROMOSOME 3, P1 CLONE: MOB24; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACAATCTAATCACGGGAAACCCTCAGGC |
| Internal bar code: | TAGAGGACCGCCCGGCCTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.3003 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATCTTCCCGGAGTTTGA |
| Suggested primer 2: | TGCTGCAAACCTTCATTCTG |