| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241132 |
| Chromosome: | chromosome_2 |
| Location: | 4517576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g100500 | CYG22 | Adenylate/guanylate cyclase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGCACACGGGGCGGCACAGGCTGCACGTG |
| Internal bar code: | TTCCGTTTTGACTGGACAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2374 |
| LEAP-Seq percent confirming: | 99.2255 |
| LEAP-Seq n confirming: | 1153 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGTGTATTGGGTGCTG |
| Suggested primer 2: | ACCTCCATGCTTGACTACCG |