| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241199 |
| Chromosome: | chromosome 6 |
| Location: | 3517274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278107 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATTCAGGGCCGCGTCCAGATCCAAATC |
| Internal bar code: | ATGCGAAAAGCTGTGATTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.6042 |
| LEAP-Seq n confirming: | 755 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGACTCTCTGCGTCGGT |
| Suggested primer 2: | CCTCCGCATACCCTCTAACA |