| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241210 |
| Chromosome: | chromosome 3 |
| Location: | 7985482 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201850 | SRH10 | (1 of 2) K10877 - DNA repair and recombination protein RAD54B (RAD54B); SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCCCTGCCGGCCGCTTCACGCCCGTCA |
| Internal bar code: | CACTTAAGCGGACTTCGTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 98.7062 |
| LEAP-Seq n confirming: | 2365 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTACGCTGTCTGGCCTTT |
| Suggested primer 2: | CCACCAAAACAACTGCACAC |