| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241234 |
| Chromosome: | chromosome 8 |
| Location: | 741290 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360500 | ERM2 | (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAAACCCACGTCCTCCCAAATCCTTGA |
| Internal bar code: | AGCTAGCTGATATGTCTTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 97.6796 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCACCTCCGACAGTGAC |
| Suggested primer 2: | CTCGCTCCTCTGTTTCCAAC |