Insertion junction: LMJ.RY0402.241234_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241234
Chromosome:chromosome 8
Location:741401
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCGACCCCACACACCTGGATGAGCGTC
Internal bar code:GTGCATCTGCGACAGGGATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:180
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AATCCACCTCCGACAGTGAC
Suggested primer 2:CTCGCTCCTCTGTTTCCAAC