| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241268 |
| Chromosome: | chromosome 3 |
| Location: | 2030876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156150 | HEL13 | (1 of 1) K14776 - ATP-dependent RNA helicase DDX10/DBP4 [EC:3.6.4.13] (DDX10, DBP4); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATCTCCTGCAGACACACGTTGGTTGCA |
| Internal bar code: | ATGCAGTGCGTGAACGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.6497 |
| LEAP-Seq n confirming: | 569 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCAAACTCCCTAGACGC |
| Suggested primer 2: | TCCGTTTTGTTGTTTCCACA |