Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.241268 |
Chromosome: | chromosome 3 |
Location: | 2030876 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g156150 | HEL13 | (1 of 1) K14776 - ATP-dependent RNA helicase DDX10/DBP4 [EC:3.6.4.13] (DDX10, DBP4); DEAD/DEAH box helicase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGAAAAGAAGGCACGGGCGCGTGCCGC |
Internal bar code: | TTTTTTGGCATTCCGTCTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 72 |
LEAP-Seq percent confirming: | 50.0 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGCAAACTCCCTAGACGC |
Suggested primer 2: | TCCGTTTTGTTGTTTCCACA |