Insertion junction: LMJ.RY0402.241268_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.241268
Chromosome:chromosome 3
Location:2030876
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g156150 HEL13 (1 of 1) K14776 - ATP-dependent RNA helicase DDX10/DBP4 [EC:3.6.4.13] (DDX10, DBP4); DEAD/DEAH box helicase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGAAAAGAAGGCACGGGCGCGTGCCGC
Internal bar code:TTTTTTTGGCATTCCGTCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:477
LEAP-Seq percent confirming:70.0348
LEAP-Seq n confirming:201
LEAP-Seq n nonconfirming:86
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAAACTCCCTAGACGC
Suggested primer 2:TCCGTTTTGTTGTTTCCACA