| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241273 |
| Chromosome: | chromosome_14 |
| Location: | 2441749 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g624800 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAGTGGGGAGGGGGGAGCGACCCTTTGGC |
| Internal bar code: | GGGGGTGAACTCACGGAGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTACATGCCGTTTCCTT |
| Suggested primer 2: | CTTGACCGCAACATACATGC |