Insertion junction: LMJ.RY0402.241303_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241303
Chromosome:chromosome 6
Location:5022480
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g280250 (1 of 1) PTHR31018:SF3 - CELL WALL MANNOPROTEIN PST1-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTCATCTACAACATGGACGCTCTGGCC
Internal bar code:CGGAATCTAGTGGACGCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:99.2908
LEAP-Seq n confirming:140
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCATCCCTGCTACTCTGC
Suggested primer 2:TATGAGTGCAGTCCAGGCAG