| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241304 |
| Chromosome: | chromosome 16 |
| Location: | 1480250 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652850 | ALG5,GTR4 | Dolichyl-phosphate beta-glucosyltransferase; (1 of 1) 2.4.1.117 - Dolichyl-phosphate beta-glucosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATCACTTCCGCCTCCCGTTGCACGGGG |
| Internal bar code: | ACGCTCGGCGATAGTGAGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.3789 |
| LEAP-Seq n confirming: | 640 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGATGTCACGGTTGGTGT |
| Suggested primer 2: | GAAACGGGGAGTGAATCTGA |