| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241325 |
| Chromosome: | chromosome 16 |
| Location: | 6846398 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675749 | SUM2,SUMO97,SUMO2 | (1 of 3) K12160 - small ubiquitin-related modifier (SUMO, SMT3); Small ubiquitin-like modifier (SUMO) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGACAAGCAATGGATGCGCAGGGGTGA |
| Internal bar code: | CCACGAAGCCCGTTCCAGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGAAGCAGACCCGACAG |
| Suggested primer 2: | CAGAGCAGCTCGAGTGAGTG |